Biologically Induced Reduction in Symmetry: A Study of Crystal Texture of Calcitic Sponge Spicules

Organisms can exert a remarkable degree of control over crystal growth. One way of achieving this is by the adsorption of specialized macromolecules on specific planes of the growing crystals. With continued growth of the crystal, the macromolecules are incorporated inside the crystal bulk. Their presence does not change the crystal structure, but creates discontinuities in the perfect lattice. Here we study in detail three unusual cases of reduction in symmetry at the level of crystal domain shapes, induced by this controlled intercalation. We examined sponge spicules, which are single crystals of Mgbearing calcite. They were specifically chosen for this study, because their morphologies d o not reflect the hexagonal symmetry of calcite. Their crystal textures (coherence lengths and angular spreads) were characterized by high-resolution Xray diffraction with well-collimated synchrotron radiation. The results are compared to analogous studies of synthetic calcite and Mg-bearing calcite. In all the selected spicules reduction in symmetry is observed in the coherence lengths among symmetry-related crystallographic directions. The reconstructed shapes of the domains of perfect structure closely match the specific spicule morphologies. The synthetic crystals show no such reduction in symmetry. Although the manner by which such exquisite control is achieved is not known, we envisage it involving a combination of oriented nucleation with either physical or stereochemically driven